human endometrial cancer cell lines kle  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Hinokitiol Exhibits Antitumor Properties through Induction of ROS-Mediated Apoptosis and p53-Driven Cell-Cycle Arrest in Endometrial Cancer Cell Lines (Ishikawa, HEC-1A, KLE)

doi: 10.3390/ijms22158268

Figure Lengend Snippet: The growth-inhibitory effect of hinokitiol on endometrial cancer cells. ( A ) Chemical structure of hinokitiol. Ishikawa ( B ), HEC-1A ( C ), and KLE ( D ) cells were treated with hinokitiol (0, 1, 5, 10, 25, and 50 µM) for 24 or 48 h; cell viability was evaluated with an MTT assay. ( E – G ) Dose–response curve and half-maximal inhibitory concentration (IC 50 ) values of hinokitiol in endometrial cancer cells. Data are expressed as the mean ± SD of three independent experiments; * p < 0.05 and ** p < 0.001 compared to the control group.

Article Snippet: The KLE human endometrial cancer cell line was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA), and it was cultured in DMEM/Ham’s F-12 (Caisson Labs, Smithfield, UT, USA).

Techniques: MTT Assay, Concentration Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Hinokitiol Exhibits Antitumor Properties through Induction of ROS-Mediated Apoptosis and p53-Driven Cell-Cycle Arrest in Endometrial Cancer Cell Lines (Ishikawa, HEC-1A, KLE)

doi: 10.3390/ijms22158268

Figure Lengend Snippet: Hinokitiol’s effect on cell-cycle-related proteins in endometrial cancer cells. Ishikawa ( A ), HEC-1A ( B ), and KLE ( C ) cells were treated with hinokitiol (0, 1, 5, 10, 25, and 50 µM) for 48 h, and the protein expression of p-P53 ( D – F ), CDK4 ( G – I ), and cyclin D1 ( J – L ) was assayed through Western blotting analysis. The differences in target proteins were analyzed with Sigma Plot, and data are expressed as the mean ± SD of three independent experiments; * p < 0.05 and ** p < 0.001 compared to the control group.

Article Snippet: The KLE human endometrial cancer cell line was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA), and it was cultured in DMEM/Ham’s F-12 (Caisson Labs, Smithfield, UT, USA).

Techniques: Expressing, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Hinokitiol Exhibits Antitumor Properties through Induction of ROS-Mediated Apoptosis and p53-Driven Cell-Cycle Arrest in Endometrial Cancer Cell Lines (Ishikawa, HEC-1A, KLE)

doi: 10.3390/ijms22158268

Figure Lengend Snippet: Hinokitiol’s effect on cell apoptosis in endometrial cancer cells. Ishikawa ( A ), HEC-1A ( B ), and KLE ( C ) cells were treated with hinokitiol (0, 1, 5, 10, 25, and 50 µM) for 48 h, and cell death was detected by using Annexin V-FITC/PI double staining with flow cytometry. The differences in the distribution of early and total apoptotic cells were analyzed with a Sigma Plot ( D – F ), and the data are expressed as the mean ± SD of three independent experiments; * p < 0.05, ** p < 0.001 compared to the control group. For ( A – C ), left lower left: healthy cells; lower right: early apoptosis; upper right: late apoptosis; upper left: necrotic cells.

Article Snippet: The KLE human endometrial cancer cell line was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA), and it was cultured in DMEM/Ham’s F-12 (Caisson Labs, Smithfield, UT, USA).

Techniques: Double Staining, Flow Cytometry, Control

Journal: International Journal of Molecular Sciences

Article Title: Hinokitiol Exhibits Antitumor Properties through Induction of ROS-Mediated Apoptosis and p53-Driven Cell-Cycle Arrest in Endometrial Cancer Cell Lines (Ishikawa, HEC-1A, KLE)

doi: 10.3390/ijms22158268

Figure Lengend Snippet: Hinokitiol’s effect on cell-apoptosis-related protein expression in endometrial cancer cells. Ishikawa ( A , J ), HEC-1A ( B , K ), and KLE ( C , L ) cells were treated with hinokitiol (0, 1, 5, 10, 25, and 50 µM) for 48 h, and the protein expression of PARP ( D – F ), Bax, Bcl-2 ( G – I ), and caspase-3 ( M – O ) was assayed with a Western blotting analysis. The differences in the target proteins were analyzed with a Sigma Plot, and the data are expressed as the mean ± SD of three independent experiments; * p < 0.05 compared to the control group.

Article Snippet: The KLE human endometrial cancer cell line was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA), and it was cultured in DMEM/Ham’s F-12 (Caisson Labs, Smithfield, UT, USA).

Techniques: Expressing, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Hinokitiol Exhibits Antitumor Properties through Induction of ROS-Mediated Apoptosis and p53-Driven Cell-Cycle Arrest in Endometrial Cancer Cell Lines (Ishikawa, HEC-1A, KLE)

doi: 10.3390/ijms22158268

Figure Lengend Snippet: Hinokitiol’s effect on autophagy-related proteins in endometrial cancer cells. Ishikawa ( A ), HEC-1A ( B ), and KLE ( C ) cells were treated with hinokitiol (0, 1, 5, 10, 25, and 50 µM) for 48 h, and the protein expression of p62/SQSTM1 ( D – F ) and LC3BII ( G – I ) was assayed with a Western blotting analysis. Data are expressed as the mean ± SD of three independent experiments; n.s.: not significant; * p < 0.05 and ** p < 0.001 compared to the control group.

Article Snippet: The KLE human endometrial cancer cell line was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA), and it was cultured in DMEM/Ham’s F-12 (Caisson Labs, Smithfield, UT, USA).

Techniques: Expressing, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Hinokitiol Exhibits Antitumor Properties through Induction of ROS-Mediated Apoptosis and p53-Driven Cell-Cycle Arrest in Endometrial Cancer Cell Lines (Ishikawa, HEC-1A, KLE)

doi: 10.3390/ijms22158268

Figure Lengend Snippet: Hinokitiol’s effect on intracellular ROSs in endometrial cancer cells. Ishikawa ( A ), HEC-1A ( C ), and KLE ( E ) cells were treated with hinokitiol (0, 1, 5, 10, 25, and 50 µM) for 48 h, and the intensity of fluorescence was assayed with H 2 DCFDA staining ( B , D , F ). Data are expressed as the mean ± SD of three independent experiments; ** p < 0.001 compared to the control group.

Article Snippet: The KLE human endometrial cancer cell line was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA), and it was cultured in DMEM/Ham’s F-12 (Caisson Labs, Smithfield, UT, USA).

Techniques: Fluorescence, Staining, Control

Journal: International Journal of Molecular Sciences

Article Title: Hinokitiol Exhibits Antitumor Properties through Induction of ROS-Mediated Apoptosis and p53-Driven Cell-Cycle Arrest in Endometrial Cancer Cell Lines (Ishikawa, HEC-1A, KLE)

doi: 10.3390/ijms22158268

Figure Lengend Snippet: Hinokitiol’s effect on the activation of the ERK1/2 pathway in endometrial cancer cells. Ishikawa ( A ), HEC-1A ( B ), and KLE ( C ) cells were treated with hinokitiol (0, 1, 5, 10, 25, and 50 µM) for 48 h, and the expression of p-ERK1/2 was assayed through Western blotting, normalized with an internal control, and quantified with the total ERK1/2 ( D – F ). Data are expressed as the mean ± SD of three independent experiments; * p < 0.05 compared to the control group.

Article Snippet: The KLE human endometrial cancer cell line was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA), and it was cultured in DMEM/Ham’s F-12 (Caisson Labs, Smithfield, UT, USA).

Techniques: Activation Assay, Expressing, Western Blot, Control